Application

This Anti-Caspase 1 Antibody is validated for use in IF, IP, WB for the detection of Caspase 1.

Western blot: 1-4 µg/mL

Immunoprecipitation: 4-8 µg/mL

Immunofluorescence: 10-20 µg/mL

Optimal working dilutions must be determined by end user.

General description

Caspase-1 (ICE, interleukin-1b-converting enzyme) is the mammalian caspase responsible for the proteolytic conversion of the proforms of interleukin-1b and IL-18 into active cytokines. Like other caspases, caspase-1 itself is synthesized as a proenzyme that is cleaved during activation into large (20 kDa) and small (10 kDa) subunits.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in PBS, pH 7.2, containing 50% glycerol, 1% BSA and 0.02% sodium azide.

Specificity

AB1871 recognizes the 45 kDa procaspase-1 as well as the 20 kDa cleaved caspase-1.